uea 1 lectin  (Vector Laboratories)

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Comparative phenotyping of surface markers and glycans in murine and human platelet-derived extracellular vesicles

doi: 10.1016/j.rpth.2026.103414

Figure Lengend Snippet: Surface glycans on l-PEVs in human and mice. The binding of fluorescein-conjugated RCA-1 (A), sWGA (B), SNA (C), MAL (D), ConA (E), and UEA lectins (F) to mouse and human l-PEVs was measured using flow cytometry. Data are represented as mean fluorescence intensity (MFI) in arbitrary units (a.u.). Orange, blue, and green lines indicate comparison between human and murine samples in resting, CRP + PAR1/4-AP stimulated (CT), or A23187 stimulated conditions, respectively. Quantification of carbohydrates in lysates of l-PEVs and platelets was performed using a microarray containing 95 lectins. (G) Comparison between total lectin binding to human and murine l-PEVs. (H) Comparison between total lectin binding to human and mouse platelets. Murine l-PEVs contained less carbohydrates, including galactose (I), N -acetylglucosamine (J), sialic acid (K), T-antigen core sugars (L), mannose (M), fucose (N), and N -acetylgalactosamine (O). Data are represented in relative fluorescence units after normalization of signal on positive and negative controls for protein binding to lectins. Data were obtained after IQR outlier test was performed. Data are mean ± SD; n = 8 to 12; ∗ P < .05, ∗∗ P < .01, ∗∗∗ P < .001, ∗∗∗∗ P < .0001. ConA, concanavalin A; GlcNAc, β-galactose and N -acetylglucosamine; MAL, Maackia amurensis ; PEV, platelet-derived extracellular vesicle; RCA, Ricinus communis agglutinin; SNA, Sambucus nigra ; sWGA, succinylated wheat germ agglutinin; UEA, Ulex europaeus agglutinin.

Article Snippet: Ricinus communis agglutinin 1, succinylated wheat germ agglutinin, Sambucus nigra , Maackia amurensis , concanavalin A, and Ulex europaeus agglutinin (all fluorescein-conjugated) were from Vector Laboratories.

Techniques: Binding Assay, Flow Cytometry, Fluorescence, Comparison, Microarray, Protein Binding, Derivative Assay

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Comparative phenotyping of surface markers and glycans in murine and human platelet-derived extracellular vesicles

doi: 10.1016/j.rpth.2026.103414

Figure Lengend Snippet: Comparison of l-PEVs generated from BCs- and fresh blood-derived human platelets. (A) Platelets were isolated from either BCs or freshly drawn citrated human whole blood. l-PEVs from unstimulated and activated (CT, A23187) conditions were analyzed using flow cytometry. Concentration of generated l-PEVs (B) and their procoagulant properties (C, D) were assessed in Trucount tubes. (E) Percentage of CD42b + l-PEVs. (F) Percentage of CD62P + l-PEVs. (G) Percentage of l-GPVI + PEVs. Surface binding of RCA-1 (H), sWGA (I), SNA (J), MAL (K), ConA (L), and UEA lectins (M). Green line depicts comparison between A23187-stimulated samples. Data are mean ± SD; n = 6; ∗∗ P < .01. BC, buffy coat; ConA, concanavalin A; CRP, collagen-related peptide; GlcNAc, β-galactose and N -acetylglucosamine; MAL, Maackia amurensis ; MFI, mean fluorescence intensity; ns, not significant; PEV, platelet-derived extracellular vesicle; RCA, Ricinus communis agglutinin; SNA, Sambucus nigra ; sWGA, succinylated wheat germ agglutinin; UEA, Ulex europaeus agglutinin.

Article Snippet: Ricinus communis agglutinin 1, succinylated wheat germ agglutinin, Sambucus nigra , Maackia amurensis , concanavalin A, and Ulex europaeus agglutinin (all fluorescein-conjugated) were from Vector Laboratories.

Techniques: Comparison, Generated, Derivative Assay, Isolation, Flow Cytometry, Concentration Assay, Binding Assay, Fluorescence

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Comparative phenotyping of surface markers and glycans in murine and human platelet-derived extracellular vesicles

doi: 10.1016/j.rpth.2026.103414

Figure Lengend Snippet: Comparison of l-PEVs generated from platelets isolated from citrated or heparinized murine blood. (A) Platelets were isolated from citrated or heparinized murine whole blood. l-PEVs from unstimulated and activated (CT, A23187) conditions were analyzed using flow cytometry. Concentration of generated l-PEVs (B) and their procoagulant properties (C, D) were assessed in Trucount tubes. (E) Percentage of CD42b + l-PEVs. (F) Percentage of CD62P + l-PEVs. (G) Percentage of GPVI + l-PEVs. Surface binding of RCA-1 (H), sWGA (I), SNA (J), MAL (K), ConA (L), and UEA lectins (M). Orange line depicts the comparison between resting samples. Data are mean ± SD; n = 6; ∗ P < .05. ConA, concanavalin A; CRP, collagen-related peptide; GlcNAc, β-galactose and N -acetylglucosamine; MAL, Maackia amurensis ; MFI, mean fluorescence intensity; ns, not significant; PEV, platelet-derived extracellular vesicle; RCA, Ricinus communis agglutinin; SNA, Sambucus nigra ; sWGA, succinylated wheat germ agglutinin; UEA, Ulex europaeus agglutinin.

Article Snippet: Ricinus communis agglutinin 1, succinylated wheat germ agglutinin, Sambucus nigra , Maackia amurensis , concanavalin A, and Ulex europaeus agglutinin (all fluorescein-conjugated) were from Vector Laboratories.

Techniques: Comparison, Generated, Isolation, Flow Cytometry, Concentration Assay, Binding Assay, Fluorescence, Derivative Assay

Journal: Pain

Article Title: Spatially resolved single-cell analysis of transcriptomic changes linked with neuropathic pain in human neuromas

doi: 10.1097/j.pain.0000000000003907

Figure Lengend Snippet: HLA-A immunohistochemistry in human neuromas. (A–C) Representative sections of HLA-A (Cy3) dual-labeling with the endothelial cell marker UEA-I (FITC, A), the axonal marker TUJ1 (FITC, B), and the immune cell marker CD45 (FITC, C) in samples of human neuromas. Scale bar: 100 μm. (D and E) Representative sections of HLA-A (Cy3) signal within nerve fascicles, marked by TUJ1 labeling (FITC) in a painful (LN4, D) and nonpainful (LN10, E) sample of human neuroma. Scale bar: 100 μm. (F) Correlation between the area of HLA-A positive staining associated with nerve fascicles (identified with TUJ1 labeling) and the intensity of pain reported by the patient. Pearson r = 0.69, P = 0.0391. FITC, fluorescein isothiocyanate; HLA, human leukocyte antigen; UEA, Ulex europeus agglutinin.

Article Snippet: HLA-A strongly colocalized with UEA-I (Vector Laboratories, FL-1061), a leptin that binds to glycoproteins expressed on the surface of endothelial cells (Fig. A).

Techniques: Immunohistochemistry, Labeling, Marker, Staining